掌握哄骗凝胶层析法测定蛋白质份子量的试验技术二、试验原理凝胶层析(gel chromatography)是20百年60年月成长起来的一种分离阐发要领其法有许多近义词如凝胶过淋、份子排阻层析、份子筛层析、凝胶渗入层析等凝胶层析是哄...
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The molecular weight estimated by gel filtration on Sephadex G-100 and SDS-PAGE is 65,000. The saccharide content of SML is 4.6% as measure by the phenol-sulfuric acid method.
Sephadex G-100凝胶层析法测得分子量为65,000道尔顿,SDS-聚丙烯酰胺凝胶电泳表明该凝集素分子仅有一个分子量为65,000道尔顿的亚基,酚-硫酸法测得总糖含量为4.6％,氨基酸组成分析表明雪山豆凝集素富含门冬氨酸,而甲硫氨酸含量甚少。
参考来源 - 雪山豆(Phaseolus sp.)凝集素的亲和层析纯化与其部分性质The purity of 97% can be achieved after two step purification of scFv including Ni metal chelating chromatography (the first) and gel filtration (the second).
经Ni离子金属螯合亲和层析、凝胶层析两步纯化,重组抗体的纯度达到97%。
参考来源 - 重组人源性抗HBsAg单链抗体在大肠杆菌中的表达、纯化与活性鉴定·2,447,543篇论文数据，部分数据来源于NoteExpress
凝胶层析法测定海芋多糖的平均相对分子质量；
Average relative molecular weight was estimated by gel column chromatograph.
方法用凝胶层析法测定116批人血白蛋白制品中多聚体的含量并对结果进行统计分析。
Method The polymers content in 116 batch of the human albumin products was determined by gelchromatography and the results were analysed by statistical method.
通过CL-6B凝胶层析分析肝素修饰物的结构变化，证实修饰后肝素的主体结构十分完整；
We tested the change of structure in heparin and chemically modified heparin with CL-6B chromatography. It is tested that the structure of them is complete.
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