After 8 hours' co-incubation at 37°C, transmigration was stopped and transmigrated cells in the lower chamber were immediately counted with the use of Synergy HT Multi-Detection Microplate Reader.
3、两种细胞于37℃共孵育8h,停止迁移试验。
参考来源 - 同型半胱氨酸诱导血管内皮氧化应激调节树突状细胞功能参与动脉粥样硬化的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
与人骨髓间充质干细胞培养上清共孵育。 流式细胞仪检测表面抗原。
The monocytes were co-incubated with supernatant from human marrow(mesenchymal) stem cells culture, and flow cytometer was used to detect surface antigen.
通过与小鼠巨噬细胞株raw264.7共孵育检测重组菌的靶向性。
The targeting property of the recombinant was detected by co-incubating the strains with murine macrophage RAW264.7.
再将RB肿瘤抗原致敏树突状细胞,与自体淋巴细胞共孵育后,能诱导出特异性杀伤肿瘤细胞的CTL。
The dendritic cells were induced by RB tumor antigen and then it cooperated with RB tumor lysates in vitro to induce antigen specific CTL which can kill the SO-RB50 target cells specifically.
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