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目的：应用无桥直接偶联模式合成靶向抗骨肿瘤偶联物甲氨蝶呤-偕二磷酸，并对其进行结构鉴定，了解其体外骨靶向性及对人成骨细胞毒性的影响。
Objective:To synthetize methotrexate-bisphosphonates conjugate by adaptation of directed couple method, and to identify its structure, bone target tropism and the effect of drug on osteoblast.
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