它对一定时间间隔内两次采样得到的图象进行运算，用最陡梯度下降法直接迭代出图象位移的估计值。

The algorithm which utilizes the Steepest Descent Method can give the estimation of displacement between two frames of image directly.

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通过检测位移与时间的关系曲线，可以免标记检测分子间的键合反应以及动力学参数(图c)。

By monitoring the shift vs. time, molecular binding events can be monitored and kinetics of the binding events can be studied without labels (Fig. C).

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