无菌条件下取正常足月剖腹产的脐带近胎儿段,剔除动静脉,取出其中的间质组织,胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
预处理时间达2星期的骨胶原表现出最高的骨明胶百分产量,然而从未处理鱼皮中得到的产率比去鳞的鱼皮得到的要高。
Ossein pretreated for a 2weeks period exhibited the maximum % yield incase of bone gelatin, while the yield from intact skin was higher than that realized incase of de-scaled skin.
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