CD34 + cells were isolated from fresh umbilical cord blood samples with immunomagnetic beads and seeded into the models to culture ex vivo without exogenous cytokines.

免疫磁珠分选脐血cd 34 +细胞，接种于各培养体系，无外源性细胞因子条件下进行培养。

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AIM: to culture in vitro umbilical cord blood mesenchymal stem cells (UCB-MSCs) by two modified methods and to observe their osteogenic differentiation potential.

目的：采用两种改良方法体外分离培养脐血间充质干细胞，并观察其成骨分化能力。

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