pcr-select subtractive hybridization pcr选择性抑制消减杂交
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Methods Improved PCR-based subtractive hybridization was used to clone the genes from apoptotic prostatic carcinoma cell line DU-145 cells induced by ATRA.
方法：应用全反式维甲酸诱导前列腺癌DU 145细胞凋亡，建立细胞凋亡模型，采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
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