• 差减杂交 - 引用次数：94

  Dominating methods in wheat gene cloning include homology-based cloning,map-based cloning,suppression subtractive hybridization,DDRT-PCR etc.

  目前,小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。

  参考来源 - 六倍体小麦基因克隆方法研究进展
  差异杂交 - 引用次数：3

  Volvariella volvacea;Subtractive cDNA library;Subtractive hybridization;Xylanase;β-Glucosidase;Chitin deacetylase;Cloning;Expression;Escherichia coli;Pichia pastoris;Purification;Recombinant enzyme;Characterization.

  草菇;cDNA削减文库;差异杂交;木聚糖酶;β-葡萄糖苷酶;几丁质脱乙酰酶;克隆;表达;大肠杆菌;毕赤酵母;重组酶;纯化;酶学性质。

  参考来源 - 草菇cDNA削减文库的构建及三种多聚糖降解酶基因的克隆与表达
• 消减杂交 - 引用次数：158

  Methods Suppression subtractive hybridization (SSH) was used to construct hypercoagulable rat cDNA subtractive library. Semi-quantitative RT-PCR was applied to identify differentially expressed genes.

  方法 采用抑制性消减杂交技术构建大鼠高血凝 c DNA消减文库 ,并用半定量 RT- PCR初步鉴定差异表达基因。

  参考来源 - 高甘油三酯血症大鼠高血凝相关基因的筛选
  差减杂交 - 引用次数：26

  In order to isolate genes differentially expressed in tissues, a variety of approaches for different display measurement have been developed and improved, such as suppressed subtractive hybridization (SSH), differential display reversed transcripted PCR (DDRT-PCR) and cDNA microarray technology.

  为了发现组织中表达的新基因，许多基于基因差异显示的技术手段，如抑制性差减杂交(SSH)，差异显示逆转录PCR(DDRT-PCR)和cDNA array杂交技术得到了应用和改进。

  参考来源 - 人类睾丸cDNA array制备及睾丸发育/精子发生相关基因表达谱系构建
  减数杂交 - 引用次数：2

  参考来源 - 新型病原体的探索研究
• 差异杂交 - 引用次数：3

  Volvariella volvacea;Subtractive cDNA library;Subtractive hybridization;Xylanase;β-Glucosidase;Chitin deacetylase;Cloning;Expression;Escherichia coli;Pichia pastoris;Purification;Recombinant enzyme;Characterization.

  草菇;cDNA削减文库;差异杂交;木聚糖酶;β-葡萄糖苷酶;几丁质脱乙酰酶;克隆;表达;大肠杆菌;毕赤酵母;重组酶;纯化;酶学性质。

  参考来源 - 草菇cDNA削减文库的构建及三种多聚糖降解酶基因的克隆与表达
  递减杂交 - 引用次数：2

  参考来源 - 应用三元递减法筛选特异性心脏生长相关基因
  扣除杂交
  消减杂交

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