...完全相同 • 限制性内切酶的剪切方式 • 平头末端(blunt ends): 在同一位置上切割双链,产生平头末端 • 粘性末端(sticky ends):交错切割,结果形成两条单链末端,这种末端的核苷酸顺 序是互补的,可形成氢键 • 主要DNA 聚合酶的作用 • 常用的DNA 聚合酶:...
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N an unpleasant finish or death (esp in the phrase come to or meet a sticky end) 不愉快的结局; 痛苦的死亡 [非正式]
When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.
它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的
If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.
如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端
If you cut and you open up a segment of DNA then you've left these sticky ends, for example and these sticky ends are capable of recognizing each other by the process of hybridization.
如果你进行切割打开一个DNA片段,就会留下,比如说,这些粘性末端,这些粘性末端能在杂交的过程中互相识别
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