A preparation method of influenza virus vaccine by using static water pressure technology and inactivation process includes the following steps: selecting toxic inoculum, * inoculation on 8-11 day-aged chick embryo and incubation at 34 deg.c, after 48-72 hr, collecting allantoic fluid, ultrafiltration and concentration, ultracentrifugation and purification, passing through column chromatography, intermediate production detection, univalent virus suspension combination, static water pressure inactivation, finished product inspection and end product packaging. Said invention combines the modern high-pressure physical technology with biological technology, not only retains complete structure of influenza whole virus, but also completely inactivates said virus, and its immunogenicity of its antigen also is not affected but raised, and so it is suitable for influenza viruses with variation and multi-variety. It is * in production process, short in production period, and low in cost.

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