COI genes of hookworm was amplified from hookworm genomic DNA by PCR and sequenced. Program MEGA was used for the sequence analysis.
采集安徽和四川两省的十二指肠钩虫,用PCR技术扩增COI基因,同时测序并用MEGA软件进行遗传差异的分析。
The positive clones were sequenced, and the sequences were analyzed by the BLAST program for homology search.
挑选阳性克隆进行测序并用BLAST软件进行同源性分析。
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