Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing.
方法对110名重庆汉族个体，采用降落式PCR和限制性片段长度多态性法（RFLP）进行基因型分析。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
One sample was found with a mutation in its first tissue microsection while not being detected in its second round of microsection was considered to be a PCR artifact.
在它的第一个组织切片中一个克隆模板的突变被发现，但是在第二个组织切片中没有检测到，被认为是在PCR克隆中核苷酸配对出现了错误。
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