Portugal coach Carlos Queiroz made four changes to the team from Monday with Pepe and RicardoCosta coming into defence, and Danny and Duda in midfield.
Portugal, semi-finalists in Germany four years ago, had RicardoCosta sent off for an off-the-ball clash with Joan Capdevila before the end but by then their fate already looked sealed.
In Spain , Valencia moved up to fifth place after snatching 3-2 win at bottom club Deportivo La Coruna, as RicardoCosta headed home in time added on before Paulo Assuncao became the second home player to be sent off.