...Japan)、BglⅡ(10 units/μl,TaKaRa,Japan)、EcoRI (15 units/ μl,TaKaRa,Japan)进行限制酶切割(restriction enzyme digestion):1μg 基因组DNA, 3μl 的10x restriction enzyme digestion buffer,3μl restriction enzyme(BamHI、BglII、 EcoRI),混匀后放入...
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...（promoter variant at position -308）基因型与LTα基因在第一个Intron的经NcoI 限制酵素切割 （restriction enzyme digestion）的基因多型性最常被拿来讨论其与气喘相关性[43,44]。
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The insert fragments and its size were confirmed by both PCR and restriction enzyme digestion. It showed that the library had a high rate of recombinant with the inserts varied from 1000 to 2000bp and the average size about 1300bp.
PCR和酶切鉴定检测插入片段大小,结果表明该文库重祖率很高,而且插入条带集中分布于1000—2000bp,平均插入大小约1300bp。
参考来源 - 花生各组织混合全长cDNA文库的构建和RGA片段的克隆·2,447,543篇论文数据，部分数据来源于NoteExpress
Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.
经pcr鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。
The length and the results of restriction enzyme digestion indicate that the amplified products are respected.
各扩增产物长度和限制性酶酶切结果表明均为预期产物。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
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