041109摘要 关键词： 口蹄疫病毒； 结构蛋白； 纯化； 复性； 活性检测 [gap=983]Key words: footandmouth disease virus；structural protein；purification；refold；activity assay
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How to refold inclusion body proteins with many pairs of disulfide bonds into natural conformation with high efficiency is a challengeable problem at present.
如何对富含二硫键的包涵体蛋白进行高效的体外复性是重组蛋白生产所面临的巨大挑战。
参考来源 - 折叠酶DsbA的制备及协助蛋白质体外复性的研究We refold the protein through quick dilution. After desalt,we purified the protein by the affinity resin.
采用快速稀释方式进行蛋白复性，采用脱盐柱脱盐后，采用亲和层析纯化制备高纯度的蛋白。
参考来源 - NDRG·2,447,543篇论文数据，部分数据来源于NoteExpress
These chaperones also refold proteins that have become unfolded.
这些伴护蛋白也会把摊开的蛋白质再折叠回去。
AIM: To refold and biotinylate HLA A2 peptide complex in vitro .
目的：可溶性HLA A2 抗原肽复合物的体外折叠复性与生物素化。
Once present in the brain it causes normal proteins to refold into the abnormal shape.
一旦在大脑中出现，它便使正常的蛋白质打褶成异常形状。
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