本发明和DNA杂交、酶联免疫吸附法(ELISA)、色谱法、常规PCR方法、随机扩增多态性PCR方法(RAPD-PCR)、限制性片断长度多态性方法(RFLP-PCR)相比更快速、准确、稳定。
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RAPD PCR 随机扩增多态性DNA
application of rapd pcr rapdpcr应用
optimal rapd-pcr conditions rapd优化反应体系
dna extraction rapd pcr dna提取
PCR-RAPD技术 PCR-RAPD technique
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The concentration of template DNA was critical to RAPD-PCR.
P CR的关键因素是模板的有效浓度。
Objective: To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR.
目的：探讨不同DNA提取方法对石斛属DNA质量及分子标记结果的影响。
RAPD reaction was carried out and programs were filtrated one by one, Optimal PCR conditions and programs were developed.
对RAPD反应条件和程序逐个进行筛选，建立了最适宜的RAPD反应体系和反应程序。
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