Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.

笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。

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Objective: to approach the optimal concentration of trypsin and the best method on the dissociation of the tissue to obtain separated primary cells.

目的：探讨胰蛋白酶消化组织块制备原代细胞的最佳浓度。

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