Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
The effects of concentration of NaOH and SDS temperature and duration of incubation on the isolation and purification of large plasmid DNA of Shigella were studied.
本实验采用碱变性的方法制备痢疾杆菌大质粒。 在制备中观察了碱、温度、时间及SDS几个因素对质粒纯度的影响。
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