Secondly, the optimal conditions of removing protein from polysaccharides by the Sevag method were studied by orthogonal experiments. The influences of the volume ratio of chloroform to normal butanol, the volume ratio of sample to chloroform–normal butanol and the time of oscillation on the deproteinization rate and loss rate of polysaccharides were investigated.
考察了氯仿与正丁醇体积比、样品与氯仿–正丁醇二元体系体积比以及混合振荡时间对蛋白去除率及多糖损失率的影响。
参考来源 - 秦巴硒菇硒蛋白多糖提取工艺研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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