Microspores culture and plant regeneration of autotetraploid Chinese cabbage were stu died . The results showed that it was necessary to isolate microspores in the late uninucleate stage.
以同源四倍体大白菜为试材，进行了游离小孢子培养的胚胎诱导条件及植株再生研究，并对再生株的染色体数进行了鉴定。
参考来源 - 四倍体大白菜小孢子植株的获得与倍性鉴定·2,447,543篇论文数据，部分数据来源于NoteExpress
Improving microspores embryogenic ability and plantlet regeneration ability from microspore-derived embryos is the key of isolated microspores culture technique.
胚胎发生能力和再生植株转化率是游离小孢子培养技术的关键问题。
The central vacuole began to disappear, and cytoplasm with abundant organelle accumulated starch in some of microspores having embryogenic ability on transfer to culture.
单核晚期小孢子经培养后，具胚状体发生能力的细胞中央液泡消失，积累淀粉，含丰富细胞器。
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