文章详细信息 关键词： Na+/H+离子逆转运蛋白基因;;宏基因组文库;;嗜盐菌;;大公古盐井 [gap=1526]Keywords: Na+/H+ antiporter gene;Metagenomic library;Halophiles;Dagong Ancient Brine Well
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The metagenomic library-based technique is an effective strategy for the utilization of un-cultured microorganisms.
宏基因组文库技术是利用未培养微生物基因资源的有效途径。
Progress on the research of metagenomic DNA purification, metagenomic library construction and screening of soil microorganisms are reviewed briefly.
对土壤宏基因组DNA的提取、宏基因组文库的构建和筛选等方面的研究现状和进展进行了简要综述。
Methods in metagenomic study, including DNA extraction, library construction and screening were discussed in this paper.
着重论述了宏基因组学的研究方法，包括DNA的提取、文库的构建以及筛选策略的选择。
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