Amplification efficiencies of the primer pairs for gene ofβ-actin,GPx4 and IDⅠwere 93.5%,91.4%and 98.4%, respectively. The melting curve analysis showed only one peak for each PCR product.
扩增β-actin、GPx4和IDⅠ基因的3对引物的扩增效率分别为93.5%、91.4%和98.4%,且PCR产物的熔解曲线显示单一峰。
参考来源 - 不同硒源对鸡原代肝细胞硒酶活性及mRNA表达的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
以上来源于: WordNet
After PCR melting curve analysis was done.
P CR反应完毕作熔解曲线分析。
Objective: To evaluate the feasibility and sensitivity of detecting the mutations of KRAS in colorectal carcinoma patients with high resolution melting curve analysis.
目的：探讨利用新的高分辨率熔解曲线分析法检测结直肠癌中KRAS基因突变的可行性。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
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