Tranmere were leading 2-1 in the second round Johnstone's Paint Trophy match when Bender was injured in the 39th minute after colliding with Accrington goalkeeper Ian Dunbavin and Tranmere's LucasAkins.
Left-back James Gibson featured in central midfield, fellow 19-year-old LucasAkins was given a rare start up front, while recent signing Trent McClenahan was given his first start as Reid attempted to plug the leaks that have bedevilled the Accies rearguard in recent weeks.