...ench ),无菌操作 箱等; 2.细胞培养箱 (cell incubator ),电热干燥箱,转 鼓培养架; 3.倒置显微镜(invert microscope),普通显微镜, 实体解剖显微镜及照相系统; 4.离心机(水平式500—4000r/min) 高速及超低温离心机。 5.
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observed with invert microscope 倒置显微镜观察
Under the invert microscope, cardiac cells growed and attached wall, like spindle, diamond or polygon, and extended pseudopodia, aggregate and had spontaneous beating.
2.1建立心肌细胞原代培养体系采用改良的Simpson法培养心肌细胞,胎盼蓝染色检测细胞的存活率大于90%,培养2~3天后倒置显微镜下观察,心肌细胞完全贴壁生长,细胞为梭形、菱形或多角形,伸出伪足,并聚集成簇,有规律的自发性搏动。
参考来源 - 附子成分次乌头碱心脏毒性及中毒机制研究·2,447,543篇论文数据,部分数据来源于NoteExpress
The cell form was observed by light invert microscope. The adhesive and invasive ability was detected by cell adhesion and migration experiments.
以细胞迁移实验和粘附实验观察细胞侵袭和粘附能力的影响。
Methods:Cell proliferation was assessed by MTT assays. Morphological changes of apoptosis were observed with invert microscope and transmission electron microscope.
方法:用三氧化二砷处理HO8910 细胞,采用MTT法检测药物对细胞的抑制作用,倒置显微镜和电镜观察细胞形态学改变。
In the invert and variable pulse electrical brushing plating technology experiment, after repair there are many Cauliflower-like organization dense and uniform under the metallurgical microscope;
在逆变脉冲电刷镀技术修复后,使用扫描电镜观察刷镀表面形貌,得到菜花头状的致密、均匀组织;
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