随后相继出现了分子信标(molecular beacons)、scorpions和杂交探针(hybridization probes),虽然这些探针不依靠DNA Taq酶有5’→3’外切酶活性,但都利用了FERT原理,最后又出现了能非特异的与双链DNA结合而发荧光的染料...
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Compared with biotinylated DNA probes and hybridization technique, PCR has a higher sensitivity.
P CR技术较体外杂交技术和DNA探针有更高的敏感性。
Conclusion in comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.
结论与双链探针比较,单链探针的应用可以明显提高RDPCR技术杂交的敏感性及特异性。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
经生物素标记探针杂交确认RANTES基因的整合,用免疫斑点印迹法对重组基因表达水平进行定量测定。
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