In this article, the latest bibliography were reviewed related to applications of detergents to in vitro expression, purification, and structural investigation of membrane proteins.
简要介绍了去垢剂在膜蛋白研究中的最新应用进展，步及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
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