Methods Duplications or deletions of?PMP22gene were detected in 113 CMT cases, 4 HNPP cases and 50 normal controls by using real-time quantitative PCR. Results (Thirty-six) of 113 CMT cases had the?
方法采用实时荧光定量PCR检测113个腓骨肌萎缩症家系先证者、4个遗传性压力易感性神经病家系先证者和50名正常人PMP22基因重复或缺失突变。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
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