本研究通过体外细胞培养技术，采用MTT法、流式细胞术（Flow Cytometry，FCM）、末端缺口原位标记法（TUNEL assay），旨在观察PYY和VES对人食管腺癌细胞SEG．1增殖及凋亡的影响，并初步探讨PYY对SEG一1生长影响的受体后作用。
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Colorimetric TUNEL Apoptosis Assay TUNEL色标法细胞凋亡检测试剂盒
Apoptosis of hepatic cells in both groups were confirmed by TUNEL assay. The brown ones were the apoptosis positive.
实验组及对照组发生凋亡的肝脏细胞均被TUNEL染色染成棕色而证实。
The fluorescence flow cytometry (FCM) DNA assay and TUNEL were applied to detect cell cycle phase and cell apoptosis. the endogenous VEGF contents were detected by ELISA method.
应用流式细胞术进行DNA倍体分析和TUNEL分析，检测两药单用与联用对细胞周期及早期凋亡的影响；
The cell viability was determined by try pan blue exclusion assay and cell cycle analyzed by flow cytometry, with the ce ll apoptosis assayed by TUNEL method.
应用锥虫蓝染色测定细胞活力、流式细胞计数分析细胞周期、TUNEL分析细胞凋亡。
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