Conclusion it is a general and effective method to clone PCR products with T-vectors.

结论t -载体法是克隆pcr产物通用而有效的方法。

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Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.

目的：构建高效表达人透明带蛋白3的真核重组表达载体。 方法：利用PCR、T- A载体克隆和亚克隆等技术。

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Even if the entire blastocyst had been implanted into a womb, it wouldn't have yielded a human clone.

WSJ: Experiment Brings Human Cloning One Step Closer

"I wasn't looking for a clone, " said Bush.

CNN: AllPolitics - Jeb Bush Jumps Into 1998 Florida Governor's Race

But Mitalipov says his process with the human cells isn't designed to generate a human clone, but rather just to create the embryonic stem cells.

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