...：双链rna；随机六聚体引物；cdna合成；高效克隆 [gap=740]key words:double-stranded rna; random hexamers;synthesis of cdna; highly efficient cloning ...
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Object: A cDNA encoding farnesyl pyrophasphate synthase (FPS) that is one of key enzymes in the synthesis of triterpene saponins from Panax notoginseng (Burkill) F. H. Chen ex C. Y. Wu & K. M.
目的：对广西中药三七主要有效成分三萜皂甙合成关键酶法昵基焦磷酸合酶的基因进行体外扩增、克隆及序列分析。
A watermelon mosaic virus 2 (WMV-2) isolate from diseased watermelon in Shanxi Province was used for cDNA synthesis of WMV-2 CP gene by RT-PCR.
本研究以从山西西瓜上分离纯化得到的西瓜花叶病毒2号（WMV-2）分离物为材料，用RT-PCR方法得到其外壳蛋白（CP）。
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