... streak culture （划线培养） successive transfer culture （继代培养） surface culture （表面培养） ...
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The flavonoid content of callus in media containing La3+ is less than that of control, and the effects of different concentrations of La3+ on flavonoid content are slight. The flavonoid content in callus is less and less, with the length of time of successive transfer culture.
本实验还发现随着继代培养次数的增多, 愈伤组织中黄酮类化合物含量也会减少。
参考来源 - 理化因子对银杏组织培养中黄酮类化合物产生影响的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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