METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.

方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。

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Selemum with given concentrations into cell cultures could effectively reduce the damages caused by serum free culture.

细胞培养液中加入一定浓度硒可有效地减轻无血清培养引起的细胞损伤。

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Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.

方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；

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