3.2 限制酶PCR(restriction enzyme PCR) 基因组DNA分别经甲基化敏感和非敏感的核酸内切酶酶切后为模板，用待检甲基化位点外侧序列为引物进行扩增。
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The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restriction enzyme analysis.
P CR检测和酶切鉴定结果表明，在水平测试的混合饲料粉中可检出牛源、羊源性成分。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
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