ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体,并验证其转录活性。
The reporter gene vector driven by SOD2 promoter will provide an experimental tool for the further study on the regulatory mechanism of the SOD2 expression.
该启动子的成功克隆和其报告基因载体的构建,为研究SOD2的基因表达调控机制提供了重要基础和工具。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
目的:构建缺氧诱导表达载体,以介导报告基因在缺氧环境下的特异、高效表达。
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