The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.

将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。

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