Objective To explore techniques of isolating embryonic stem-like cells (ESLCs) from mouse blastocyst inner cell mass and the utilization of ESLCs to generate chimerical mice.
目的探讨分离小鼠囊胚内细胞群类胚胎干细胞及用于制作嵌合体小鼠的方法及应用价值。
Results Acquired 16 passages in mouse and 7 passages in human and established the culture system of human embryonic stem-like cells.
结果分别获得了传16代的胎鼠成纤维细胞和传7代的人胎儿成纤维细胞，建立了人胚胎干细胞饲养层的培养体系。
He and Andras Nagy from the University of Toronto used the technique in both mouse and human skin cells and found the reprogrammed cells behaved just like embryonic stem cells.
他和加拿大多伦多大学的安德拉斯·纳吉技术在老鼠和人类的皮肤细胞上使用了这项技术，并发现重编程的iPS细胞表现出和胚胎干细胞一样的特性。
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