Method:Human nucleus pulposus cells acquired from degenerated lumbar intervertebral discs were cultured by monolayer culture and identified.
方法:单层培养并鉴定人退变腰椎间盘髓核细胞。
参考来源 - 腺相关病毒载体介导的TIMP1对人退变腰椎间盘髓核细胞蛋白多糖的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
方法用酶消化、分离鸡胚关节软骨细胞，体外单层培养，收集细胞培养液。
To study the methods for isolation and culture of articular chondrocyte and to observe the phenotypic expression of the monolayer cultured cells at a high density.
探讨兔关节软骨细胞的分离、培养方法，观察单层高浓度培养时细胞表型表达情况。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
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