The map makes effective tools for QTL analysis, marker-assisted selection (MAS) in breeding programs and cloning of the genes responsible for various phenotypes (map-based cloning).

该图谱的建立为进行基因定位、分子标记辅助选择抗旱性育种和抗旱相关基因的图位克隆奠定基础。

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However the former cloning methods such as map-base cloning or transposon tagging which based on forward genetics can only cloned one or several genes at the same time.

以往的基因克隆方法如图位克隆法、转座子标签法等大都是建立在正向遗传学的基础上，这些方法只适用于一个或少数基因，应该说是零敲碎打的策略。

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In this dissertation we focused on the function of genes associated with anther and pollen development including map-based cloning of Arabidopsis genes from male sterile mutants.

本论文以正向遗传学的方法研究花药及花粉发育过程的基因功能，主要利用图位克隆的方法从拟南芥雄性不育突变体中克隆相应的基因。

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