Methods The compounds were isolated by silica gel, ODS, and macroporous resin column chromatography and their structures were determined by means of spectral analysis.
方法利用正相、反相硅胶柱和大孔树脂柱色谱分离，并通过波谱技术进行结构鉴定。
The product of fermentation was separated with macroporous resins, and then purified by the silica-gel column chromatography and recrystallization. The pure product was assayed by NMR, IR and MS.
发酵产物经大孔吸附树脂、硅胶柱层析及重结晶等方法制备出牛蒡子苷元纯品，并采用核磁共振（NMR）、红外（IR）、质谱（MS）等方法对产物进行了鉴定。
Method: Isolation and purification of the trace constituents were carried out mainly by solvents extraction and macroporous adsorbing resin and silica gel column chromatography.
方法：用纯净水提取，溶剂萃取，大孔吸附树脂和硅胶柱色谱分离等方法分离并纯化亚麻根的微量成分，通过波谱分析鉴定化合物的结构。
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