To understand intron 15 of human LDL receptor gene, the DNA fragments from exon 15 to exon 16 and the 3' end of intron 15 were amplified with long chain PCR and anchored PCR.

为了解人类LDL受体基因内含子15的遗传背景，利用长链PCR和锚定PCR分离了LDL受体基因外显子15-内含子15-外显子16和内含于15的3’末端片段。

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Previous estimates said there were only 400 HoFH patients in the U.S. but included only those people confirmed to have two defective copies of the LDL receptor gene.

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