The principle of two-photon laser scanning fluorescence microscopy and its applications are reviewed.
文章对双光子激光扫描荧光显微镜的原理及其应用进行了综述。
Objective To explore the conditions under which fluorescence intensity is determined in confocal laser scanning microscopy.
目的对应用激光扫描共聚焦显微镜进行荧光强度的测定条件做了详细的分析。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
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