Subsequently competent cells were prepared and transformed. After large-scale preparation of plasmid DNA, recombined adenoviruses Ad5F35-IL24 were generated with homologous recombination by cotransfection of 293 cells with a couple plasmids.
制备感受态细胞,进行转化,大量制备质粒DNA;之后用双质粒共转染293细胞,产生重组腺病毒Ad5F35-IL24。
参考来源 - 腺病毒介导IL·2,447,543篇论文数据，部分数据来源于NoteExpress
Conclusion This simple method with high yield can be used for laboratory research and large-scale preparation.
结论本法简单、产率较高，可用于实验室研究和大规模制备。
At present, many problems about application and industrialization of CNTs need to be solved, and the technology of large-scale preparation of CNTs has not been well developed.
目前碳纳米管的应用及产业化过程还存在诸多问题，碳纳米管的规模化制备技术尚未成熟。
In this paper, the advantages of microreactor compared to large-scale reactors, the preparation and applications of the microreactors fabricated with zeolites and zeolite membranes were reviewed.
综述了微反应器的优点以及运用沸石及其膜组装微型反应器的制备方法、研究进展及应用前景。
应用推荐