摘要04 关键词： 猪生殖与呼吸综合征病毒；感染性克隆；体外转录；转染；RNA印迹[gap=1774]Key words: PRRSV;infectious clone;in vitro transcription;transfection;Northern blot
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RNA in vitro transcription RNA体外转录
in vitro transcription assay 体外转录分析
double in vitro transcription 双轮体外转录
infectious in vitro transcription 感染性体外转录本
in vitro transcription and transfection 体外转录和转染
It can be directly transfected into cells to achieve a high level of exogenous gene expression without in vitro transcription.
与其相比,质粒型复制子载体可通过真核启动子(如CMV)启动转录,无需体外转录可直接转染到细胞中实现高水平外源基因的表达。
参考来源 - XJ·2,447,543篇论文数据，部分数据来源于NoteExpress
Actin was also reported to co-purify with RNA polymerase II and to be required for efficient in vitro transcription by RNA polymerase II.
肌动蛋白还能与RNA聚合酶II共纯化，对RNA聚合酶II体外的有效转录起到作用。
Conclusion: Expression of TTF1 in vitro can be used in the further research of interaction of TTF1 and its DNA response elements and others transcription factors.
结论：能方便地获得TTF1体外翻译蛋白，为进一步研究TTF1蛋白与相应的DNA反应元件及其他转录因子的相互作用奠定了基础。
Methods EOLA1 protein was expressed in vitro through couple transcription and translation. The products were detected by gel electrophoresis.
方法通过偶联转录翻译系统体外表达EOLA1蛋白，并用变性凝胶电泳进行产物检测；
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