METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
Objective: To construct a replication-competent adenovirus vector carrying human endostatin gene, looking for an effective method for the therapy of colon cancer.
目的：构建携带内皮抑素基因的增殖型腺病毒载体，寻找结肠癌的有效治疗方法。
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