After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
包涵体蛋白经含8m尿素的结合缓冲液溶解后,用金属亲和层析法进行纯化,获得了高纯度的目的蛋白。
Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity.
链霉素亲和素是从链霉亲生物素蛋白中分离出来的53,000道尔顿的同源四聚体(3),每一个链霉素亲和素的亚基可以与维生素形成高亲和力非共价结合。
Immobilized metal affinity chromatography (IMAC) is a well-established protein separation technique experiencing expanded use based on new, high-performance analytical phases.
固定化金属离子亲和色谱(IMAC)作为一种新型高效色谱分离模式,已经被广泛应用于蛋白质的分离分析。
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