Methods: The manifestations of cerebral lesions and apoptosis in the brain ischemic rats with hyperglycemia were investigated by HE and TUNEL staining.
方法：采用大鼠高血糖全脑缺血模型，用HE和TUNEL染色，对比检测脑细胞损伤和凋亡。
HE and silver staining, observed with light microscope and apoptosis were detected by TUNEL.
实验后采用HE染色、银染色，光镜观察；TUNEL法检测凋亡。
Meanwhile, the pathological changes of liver was observed under light microscope after he staining, and hepatocyte apoptosis was detected by the TUNEL method.
采血后取肝组织，常规石蜡包埋，切片做HE染色观察肝组织的病理变化，采用TUNEL法检测肝细胞的凋亡。
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