The cells had been frozen and thawed out before being used, to simulate the conditions they would be kept in if stored for human patients.
干细胞在使用前都事先经过冰冻和解冻处理，以模拟用于人类目的的干细胞的存放条件。
The cells were passaged by mechanical methods, frozen and thawed by general procedure.
采用机械方法传代，常规方法冻存和复苏。
Objective To evaluate the effects of human frozen thawed decidua monolayer cells on mouse embryo development in a co culture system.
目的探讨冻融的人早孕蜕膜单层细胞对小鼠胚胎发育的影响。 方法蜕膜单层细胞解冻后接种获得冻融蜕膜单层细胞；
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