The fluorescence polarization degree of fluorescence probe DPH labelled in cells was calculated from corrected fluorescence components measured by steady-state fluorescence technique.
用稳态荧光技术测得经过校正的荧光成分,由此算出用DPH标记的细胞膜的偏振度。
This present paper dealt with the method of study on the fluidity of mitochondrial membrane from housefly flight muscles using DPH as a fluorescence probe.
本文探讨了用DPH作为荧光探剂,研究家蝇飞翔肌线粒体膜脂流动性的方法。
METHODS: The fluidity of membrane lipid regions of splenocytes was determined by the fluorescence polarization technique using 1, 6-diphenyl-1, 3, 5-heatriene (DPH) as a fluorescence probe.
方法:以1,6-二苯基,1,3,5-己三烯(DPH)作为荧光探针,利用荧光偏振法测定脾细胞膜脂质区的流动性。
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