The double immunodiffusion test and ELISA were used to test the titer of the antiserum. The specificity of antibody was detected by SDS PAGE and ELIB (Enzyme linked immune blotting).
采用多途径免疫法制备单特异性抗血清，琼脂扩散试验和ELISA测定抗体的效价，SDS PAGE和ELIB检测抗体的特异性。
Double immunodiffusion assay was used to identify the type and subclass of monoclonal antibody. ELISA and double immunodiffusion assay were used to determine the specificity.
采用免疫双扩法鉴定单克隆抗体类型，ELISA法、免疫双扩法鉴定单克隆抗体的特异性。
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