This research provided techniques for the application, conservation in vitro of excellent germplasm and reduction of heredity variance of autotetraploids of Plalycodon grandiflorum.
本研究为优良同源四倍体桔梗的推广应用和优良种质离体保存、减少遗传变异提供了技术依据。
Subculture is an important link in the process of the tissue culture and the long-term subculture is the essential method for conservation in vitro of germplasm.
在植物组织培养过程中，继代培养是其中的重要环节，而长期继代则是种质资源离体保存的必要手段。
Restricting conservation and cryopreservation are two main methods of plant germplasm in vitro preservation.
限制生长保存和超低温保存是植物种质离体保存的两种主要方法。
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